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Question : Highlight the protocol for production of virus free meristem tip culture.

Answer»

SOLUTION :Protocol for virus free meristem tip culture
`DARR`
Apical meristem tip with 1or 2 leaf
primordia are excised in STERILE
condition from the explant
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In vitro culture in 10 ml of solid MS medium
supplemented with GROWTH hormones
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Cultures are maintained at `24pm1^(@)C` in
dark for 3 days followed by NORMAL
illumination of 2400Lux
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Plantlets developed from meristem-
tip culture after organogenesis process,
transferred to hardening process
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Transferred to field condition


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