Question : How can DNA segments, separated by gel electrophoresis, be – InterviewSolution

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1.	Question : How can DNA segments, separated by gel electrophoresis, be visualized and isolated?
Answer» Solution :DNA segments, that have been SEPARATED on an AGAROSE gel by electrophoresis are transferred and permanently AFFIXED to a nylon membrane, a process called southern blotting. The DNA bands are invisible to the EYE so that they are flagged with some kind of tag that one can visualize. Therefore, a solution containing radioactive probe is spread over the membrane so that they bind to complementary DNA sequences and then can be VISUALIZED by autoradiography.

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