Question : How will you avoid the growing of microbes in nutrient medi – InterviewSolution

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1.	Question : How will you avoid the growing of microbes in nutrient medium during culture process? What are the techniques used to remove the microbes?
Answer» Solution :The microbial GROWTH in culture medium can be overcome by AUTOCLAVING the medium at `121^(@)C` (15 psi) for 15 to 30 minutes. Chemical sterilization using chemicals, sterilizaing using UV radiation. ALCOHOLIC sterilization using ethanol, autoclaving and filtration etc., are the various techniques used to remove MICROBES.

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