Question : Insulin getting assembled into a mature form was the major challenge in commercial insulin production by rDNA technology. How did Eli Nilly Company found a solution to this problem?

Question : Insulin getting assembled into a mature form was the major

1.	Question : Insulin getting assembled into a mature form was the major challenge in commercial insulin production by rDNA technology. How did Eli Nilly Company found a solution to this problem?
Answer» Solution :Eli Lilly company prepared two DNA SEQUENCES corresponding to A and B CHAINS of human insulin. Introduced them in plasmids of E.coli to produce insulin chains. Chains A and B were produced separately, extracted and COMBINED by CREATING disulfide BONDS to form human insulin.