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Question : Mention the different steps of process of recombinant DNA technology.

Answer»

Solution :a. ISOLATION of DNA
b. Fragmentation of DNA by RESTRICTION endonucleases.
c. Isolation of a desired DNA fragment.
d. Ligation of the DNA fragment into a vector.
e. INSERTION of recombinant DNA into the hast.
f. Culturing the HOST cells on a suitable medium at a large scale.
g. Extraction of the desired gene product.
h. Down stream processing of the products as FINISHED product, ready for marketing.


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