Question : Name any two cloning vectors. Describe the features required to facilitate clon-ing into a vector .

Question : Name any two cloning vectors. Describe the features require

1.	Question : Name any two cloning vectors. Describe the features required to facilitate clon-ing into a vector .
Answer» Solution :Plasmid and bacteriophages are the commonly used vectors .The FOLLOWING FEATURES are required to facilitate cloning in a vector . i. ORIGIN of replication (oir) ii. Selectable market iii. Cloning (recognition) site iv. Small size of vector i. Origin of replication (oir) : This is a sequence from where replica-tion starts and any piece of DNA when linked to this sequence can be mode to replicate within the host cells . The target DNA should be CLONED in a vector whose origin supports high copy number. ii. Selectable marker :In addition to .ori. the vector REQUIRES a se-lectable marker which helps in identifying and eliminating non transformants and selectively permitting the growth of the trans-formants. Transformation is a procedure through which a piece of DNA is introduced in a host bacterium. iii.Small size of vector : The mechanisms to deliver genes of our interest into variety of plants are now used to deliver desirable genes into plant cells. So once a gene or a DNA fragment has been ligated into a suitable vector, it is transferred into a bacterial plant or animal host .