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Question : One of the methods by which recombinant DNA can be introduced into host cell is by 'heat shock' treatment (electro-poration). Write down other three important methods by which we can introduce alien DNA into host cells .

Answer»

Solution :i. Micro-injection : Recombinant DNA is DIRECTLY injected into the nucleusof animal cell.
ii. Biolistics or gene gun : Cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA.
iii.By disarmedpathogen vectors :These vectors are allowed to INFACT the cell, which TRANSFERS the recombinant DNA into the host .


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