InterviewSolution
Saved Bookmarks
| 1. |
Question : Polymerase Chain Reaction is the synthesis of multiple copies of gene of interest in vitro using two sets of primers and DNA polymerase enzyme. Commonly used DNA polymerase for PCR reaction is Taq polymerase. Write the significance of Taq polymease enzyme. |
|
Answer» Solution :Taq polymerase is a thermostable DNA polymerase enzyme isolated from bacterium-Thermus aquaticus, which REMAINS ACTIVE during high TEMPERATURE and induced denaturation of singl stranded DNA. Taq polymerase synthesises the DNA strands complementary to the DNA template srand, i.e., primer extension. |
|