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Question : Recombinant DNA technology can be accomplished only if we have the following key tools i.e., Restriction enzymes, polymerase enzyme. Ligase and vecors. State the functions of (a) Ligase (b) Restriction enzymes. |
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Answer» Solution :(a) Ligases: It is anenzyme USED to join the DNA FRAGMENTS. It is a moleular suture or molecular glue or joiningenzyme which is commonly used in rDNA technology (b) RESTRICTION enzyme: Enzymes which cut DNA at specific SITES into fragmentscontaining identifiable genes/It is KNOWN as biological scissors/molecular scissors. |
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