Question : Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at 'specific-recognition sequence'. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?

Question : Restriction enzymes that are used in the construction of re

1.	Question : Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at 'specific-recognition sequence'. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
Answer» SOLUTION :For reconstruction of recombinant DNA, restriction enzyme NEEDS to make cut at specific SITE so that both vector DNA and insert DNA have the same sticky ends. U restriction enzyme cuts at random sites, the two types ofDNAs will not have sticky ends and recombinant DNA molecule will not be formed.