Question : Rinku with a circular DNA contains sequence 5' larr GGATTCC to3' 3' larr CCTTAAGG to5' She wishes to add a new segment of DNA into it. a. Identify the technology she planned. b. Suggest the specific enzyme to make a cut in the DNA with above sequence. c. Name the ctegory of enzyme your suggested. d. How this enzyme identifies the sequence? e. Draw the cut ends of the DNA with sequence.

Question : Rinku with a circular DNA contains sequence 5' larr GGATTC

1.	Question : Rinku with a circular DNA contains sequence 5' larr GGATTCC to3' 3' larr CCTTAAGG to5' She wishes to add a new segment of DNA into it. a. Identify the technology she planned. b. Suggest the specific enzyme to make a cut in the DNA with above sequence. c. Name the ctegory of enzyme your suggested. d. How this enzyme identifies the sequence? e. Draw the cut ends of the DNA with sequence.
Answer» Solution :a. RECOMBINANT DNA technology b. Restriction ENZYMES . e.g. EcoRI c. Restriction ENDONUCLEASES d. Each restriction endonucleases recognises aspecific palindromci nu-clecotide SEQUENCE.