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Question : State the principle underlying 'gel electrophoresis' and mention the applica-tion of this technique in biotechnology .

Answer»

Solution :The principle UNDERLYING gel electrophoresis isthat when a bio-molecule like DNA is placed in a gel MATRIX (agarose gel) under an electric field , the molecule separates according to theircharge and size. It Is widely used in the separation of FRAGMENTS produced as a result of restriction endonuclease digestion.


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