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Question : Summarise the events in sequence when a mixture of plasmid vector and human DNA is digested with restriction enzyme EcoRI is supplemented with enzyme DNA ligase. |
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Answer» SOLUTION :(a) Enzyme fcaR! will introduce sticky end cuts in plasmid vector and will cleave HUMAN DNA into SEGMENTS with similar sticky ends. (b) With the help of DNA LIGASE some plasmid molecules will form recombinant DNA by joining human DNA segment. |
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