Question : Summarize major events of DNA finger printing technique.

1.	Question : Summarize major events of DNA finger printing technique.
Answer» Solution :1) Dr.Alec Jeffery.s developed the techniques of DNA FINGERPRINT, an attempt to identify DNA marker for inherited diseases. 2) DNA finger printing uses short nucleotide repeats CALLED variable number tandem repeats (VNTRs) as markers VNTRs vary from person to person and are inherited from one generation to the next. Methodology and Technique DNA is isolated and extracted from the cell or tissue by centrifugation. By the PROCESS of polymerase chain reaction (PCR) many copies are PRODUCED. This step is called amplification. DNA is cut into small fragments by treating with restriction endonuclease DNA fragments are separated by agarosegel electophoresis The repeated DNA fragments are visualized under ultraviolet radiation after applying suitable dye The DNA is transferred from electrophoresis plate to NYLON membrane sheet. This is called southern blotting Probes are now added which bind to specific nucleotide sequence that are complementary to them, this is called hybridisation. The hybridised DNA fragments are detected by auto radiography. They are observed as dark bands on X - ray film.

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