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Question : The recognition sequencesof restriction enzyme 'A' is GGCC and that of 'B' is GAATTC. Which statement among the following is true regarding the probable frequency of cutting sides in a genome.

Answer»

Both A and B cut EQUALLY frequently
A cuts once every 676 nucleotides and B 1024 nucleotides
A cuts once every 256 nucleotides and 4096 nucleotides
A cuts once every 256 nucleotides and B 1024 nucleotides

Solution :There are four nucleotides, i.e. A, T, G and C. In case of A enzyme, it is four nucleotides sequences cutter. Therefore, the probable FREQUENCY of fragments produced by A is `4^(4) = 256` Nucleotides. Similarly, with B enzyme, the NUMBER of nucleotides. Similarly by it is `4^(6) = 1024` nucleotides.
THus, A cut (s) at every 256 nucleotides and B produces cut at 1024 nucleotides in sequences.


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