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Question : The role of DNA ligase in the construction of a recombinant DNA molecule is

Answer»

Formation of phosphodiester bond between TWO DNA fragments
Formation of hydrogen BONDS between sticky ends of DNA fragments
Ligation of all purine and pyrimidine bases
None of the above

Solution :Formation of phosphodiester bond between two DNA fragments
The role of DNA ligase in the construction of a RECOMBINANT DNA molecule is formation of phosphodiester bond between two DNA fragments. It seals the GAP between the DNA fragments. DNA ligase is ALSO called as molecular glue.


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