Question : There was great excitement around the world when the sheep 'Dolly' was cloned using a nucleus derived from an adult cell of its 'mother' which was them transplanted into an enucleated egg. There is also excitement when it is announced that genes causing human diseases, like muscular dystrophy, have been cloned. Which statement about these two examples of cloning is correct ?

Question : There was great excitement around the world when the sheep

1.	Question : There was great excitement around the world when the sheep 'Dolly' was cloned using a nucleus derived from an adult cell of its 'mother' which was them transplanted into an enucleated egg. There is also excitement when it is announced that genes causing human diseases, like muscular dystrophy, have been cloned. Which statement about these two examples of cloning is correct ?
Answer» They both INVOLVE cutting a piece of DNA from the genome One involves the cloning of a nucleus and the other is the cloning of a piece of DNA They both produce products genetically IDENTICAL to the original donor of cellular material They raise no ETHICAL questions Solution :The sheep 'Dolly' was produced by transferring entire nucleus into an encleated cell white cloning of genes like genes of muscular dystrophy involves cutting the DNA piece containing the particular gene and transferring its through a vector or through other methods into a host cells. Tools of recombinant DNA technology like restriction ENZYMES, etc. are not used in first experiment while they are essential for the second. In the first experiment,cell express all the genes of the donor while cells in the second experiment, express only the desirable gene that is transferred.