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Question : To prove that DNA is the genetic material, which radioactive isotopes were used by Hershey and Chase (1952) in their experiments?

Answer»

`.^(35)S and .^15 N`
`.^(32)p and .^35 S`
`.^(32)p and .^15 N`
`.^(14)N and .^15 N`

Solution :Hershey and Chase experiment is BASED on the FACT that DNA contains PHOSPHORUS and similarly sulphur is present in proteins but not in DNA. They incorporated RADIOACTIVE isotope of phosphorus`(overset(32)P)` into phage DNA and that of sulphur `(overset(35) S)` into proteins of a separate phage culture. Viruses grown in the presence of radioactive proteinof radioactive phosphorus contained DNA contained radioactive DNA but not radioactive protein because DNA contains phosphorus but proteins do not . Similarly , viruses grown on radioactive sulphur CONTAIN radioactive protein but not radioactive DNA because DNA does not contain sulphur.


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