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Question : What are the optimal conditions that favours the induction of callus from nutrient medium? |
| Answer» Solution :After the inoculation of explant in the nutrient medium supplemented with AUXINS and INCUBATED at `25^(@)Cpm2^(@)C` in an alternate light and dark period of 12 HOURS with light intensity of 1000 lux, induces CELL division leading to the development of callus from the surface of explant. | |