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Question : What do you know about the word pBR332? |
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Answer» <P> Solution :pBR 322 plasmid and most widely used as cloning vector , it CONTAINS 4361 base pairs. In pBR , p denotes plasmid , B and R respectively the names of scientist the names of scientist Boilver and Rodriguez who DEVELOPED this plasmid . The number 322 is the number of plasmid developed from their laboratory. It contains `"amp"^R and "tet"^R` two different ANTIBIOTIC resistance genes and recognition sites for several redistricting enzymes. (Hind III, EcoRI, BamH I , Sal I, Pvu II , Pst I and Cla I) , ori and antibiotic resistance genes. Rop codes for the proteins involved in the replication of the plasmid.
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