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Question : What is polymerase chain reaction? Name the bacterium from which the polymerase enzyme used in this technique is obtained. Write the schematic representation of this technique. |
Answer» Solution : POLYMERASE CHAIN reaction- In this reaction, multiple copies of the gene (or DNA) of interest is SYNTHESISED in vitro using two sets of primers (SMALL chemically synthesised oligo NUCLEOTIDES that are complementary-to the regions of DNA) and the enzyme DNA polymerase. Polymerase enzyme is obtained from a bacterium Thermus aquaticus. |
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