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Question : What will happen if- i) A plasmid vector is digested with Eco RI at a single site ii) A sample of human DNA is digested with Eco RI. iii) The two samples (plasmid and human DNA) are allowed to hybridise in presence of DNA ligase.

Answer»

Solution :i) It will produce two sticky ENDS.
II) Human DNA pieces will also form sticky ends.
iii) Some MOLECULES will form with the pieces of human DNA inserted into the plasmid VECTOR at the ECO RI site.


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