InterviewSolution
Saved Bookmarks
InterviewSolution
| 1. |
Question : What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing ? |
| Answer» Solution :In the absence of ANTIBIOTIC, there will be no pressure on RECOMBINANTS to retain the PLASMID (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a METABOLIC burden to the microbial cells, will thus tend to lose the plasmid. | |