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Question : Whether PCR can be done for RNA molecules? Yes or No? Explain. |
Answer» Solution :The PCR technique can ALSO be used for amplifications of RNA in which case it is refered to as REVERSE transcription PCR (RT-PCR). In this process the RNA molecules (mRNA) must converted to complementary DNA by the enzyme reverse transcriptase. The CDNA then serves as the TEMPLATE for PCR. | |