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Rajesh was doing gel electrophoresis to purify DNA fragments. Given below is the sketch of the observations of the experiment performed by him.(a) At which end he would have loaded the samples and where ? (b) Analyse the reason for different positions taken up by the DNA bands. (c) Elaborate the step he would have followed to visualize DNA bands. |
Answer» (a) He would have loaded the samples near end A, in the wells. (b) The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel. Hence, the smaller type fragment size, the farther it moves (c) After staining the DNA with ethidium bromide followed by exposure to UV radiations the DNA bands appear coloured. |
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