Raju is a diabetic parient who takes insulin injections regulary. The

1.	Raju is a diabetic parient who takes insulin injections regulary. The insulin used by such patients is producted by genetically engineered organisms . Write the different steps involved in the production of insulin by genetic engineering.
Answer» (1) Preparation of DNA sequences corresponding to A&B chain of human insulin. (2) Introduce them in plasmid of E.coli. (3) Products of A&B Chains are separated. (4) Combine A&B chains by creating disulphide bonds

Raju is a diabetic parient who takes insulin injections regulary. The insulin used by such patients is producted by genetically engineered organisms . Write the different steps involved in the production of insulin by genetic engineering.

Answer»

(1) Preparation of DNA sequences corresponding to A&B chain of human insulin.

(2) Introduce them in plasmid of E.coli.

(3) Products of A&B Chains are separated.

(4) Combine A&B chains by creating disulphide bonds