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Recombinant insulin is produced at 100 mg/l by E. coli at a cell concentration of 1 g/l. Calculate the volume of reactor (size of the fermentor) needed to produce 1 kilogram of insulin in the following conditions:(a) When the cell concentration is 1 g/l and insulin production is 100 mg /l. (b) When the cell concentration is 50 g/l and insulin production is 100 mg /l. (c) When the cell concentration is 50 g/l and insulin production is 500 mg /l |
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Answer» (a) Insulin production is 100 mg/L; so fermentor volume needed for 1 Kg of insulin is 1 Kg /100mg = 1000, 000mg/100,g = 10,000mg = 10,000L. (b) Cell concentration is increased 50 times, we need 200 L reactor. (c) Insulin yield per litre of culture is 500 X 50 = 25, 000 mg / L which is 25 gram/L. We need a 40 L reactor (1000g/25g) . |
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