Short note on :Bt cotton.

1.	Short note on :Bt cotton.
Answer» 1. Bt cotton is well known example of insect resistant transgenic plant which is engineered with a gene from B. thuringiensis. 2. ‘cry’ gene present in B. thuringiensis produces a protein that forms crystalline inclusions in bacterial spores. 3. When insect ingests it, because of high pH and the proteinase enzymes present in insect’s midgut, the crystalline inclusions are hydrolyzed to release the core toxic fragments. 4. This toxin causes midgut paralysis and disruption of midgut cells of insect. 5. Bt toxin acts against many species of Lepidoptera, Diptera and Coleoptera insects.

Answer»

1. Bt cotton is well known example of insect resistant transgenic plant which is engineered with a gene from B. thuringiensis.

2. ‘cry’ gene present in B. thuringiensis produces a protein that forms crystalline inclusions in bacterial spores.

3. When insect ingests it, because of high pH and the proteinase enzymes present in insect’s midgut, the crystalline inclusions are hydrolyzed to release the core toxic fragments.

4. This toxin causes midgut paralysis and disruption of midgut cells of insect.

5. Bt toxin acts against many species of Lepidoptera, Diptera and Coleoptera insects.

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