Short note on :Insulin.

1.	Short note on :Insulin.
Answer» 1. Insulin is a peptide hormone produced by β - cells of islets of Langerhans of pancreas. 2. Insulin is essential for the control of blood sugar levels. 3. Disease Diabetes mellitus is caused due to inability to make insulin. 4. Insulin was discovered by Sir Edward Sharpey Schafer (1916) while studying Islets of Langerhans. 5. Hakura et al (1977) chemically synthesized DNA sequence of insulin for two chains A and B and separately inserted into two PBR322 plasmid vector. Gilbert and Villokomaroff, 1978 produced insulin production using r-DNA technology. 1. The recombinant plasmids, containing insulin gene inserted by the side of β-galactosidase, were transferred into E. coli host. 2. The host produced penicillinase pnd pre-pro insulin. 3. Insulin is later separated by trypsin treatment.

Answer»

1. Insulin is a peptide hormone produced by β - cells of islets of Langerhans of pancreas.

2. Insulin is essential for the control of blood sugar levels.

3. Disease Diabetes mellitus is caused due to inability to make insulin.

4. Insulin was discovered by Sir Edward Sharpey Schafer (1916) while studying Islets of Langerhans.

5. Hakura et al (1977) chemically synthesized DNA sequence of insulin for two chains A and B and separately inserted into two PBR322 plasmid vector.

Gilbert and Villokomaroff, 1978 produced insulin production using r-DNA technology.

1. The recombinant plasmids, containing insulin gene inserted by the side of β-galactosidase, were transferred into E. coli host.

2. The host produced penicillinase pnd pre-pro insulin.

3. Insulin is later separated by trypsin treatment.

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