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Some of the steps involved in production of humulin are given below. Choose the correct sequence (i) Synthesis of insulin gene artificially (ii) Culturing recombinant E. coli in bioreactors (iii) Purification of humulin (iv) Insertion and human insulin gene into plasmid (v) Introduction of recombinant plasmid into E. coli (vi) Extraction of recombinant gene product from E. coli.A. ii,iiv,iii,v,viB. I,iv,vii,vi,iiiC. I,iii,v,vi,ii,ivD. iii,v,ii,vi,iv

Answer» Correct Answer - B
Some of the steps involved in porduciton of humulin are given below .The correct sequence are :
(i) Synthisis of insulin g gene artificially
(iv) insertion of human insulin gene in to plasmid
(v) introduction of recombinant plasmid in to e coli
(ii) extraction of recombinant gene product from e coli
(iii) pruification of humulin (human insulin)


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