Stanley cohen and Hebert Boyer constructed r DNA by using plasmid and

1.	Stanley cohen and Hebert Boyer constructed r DNA by using plasmid and antibiotic resistant gene.1. Name the slicing and splicing enzyme used.2. What is the significance of slicing enzyme in rDNA technology?
Answer» 1. Slicing enzyme – Restriction endo nuclease Splicing enzyme-DNA ligase 2. significance of slicing enzyme in rDNA technology: It is used to cut within DNA sequence. Same restriction enzyme is used to cut both vector DNA and foreign DNA.

Stanley cohen and Hebert Boyer constructed r DNA by using plasmid and antibiotic resistant gene.1. Name the slicing and splicing enzyme used.2. What is the significance of slicing enzyme in rDNA technology?

Answer»

1. Slicing enzyme – Restriction endo nuclease Splicing enzyme-DNA ligase

2. significance of slicing enzyme in rDNA technology:

It is used to cut within DNA sequence.
Same restriction enzyme is used to cut both vector DNA and foreign DNA.

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Stanley cohen and Hebert Boyer constructed r DNA by using plasmid and antibiotic resistant gene.1. Name the slicing and splicing enzyme used.2. What is the significance of slicing enzyme in rDNA technology?

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