1.

The action of alcohol during Gram staining isO Allows the colorO It adds colorO Decolorises the cellsO None of these​

Answer»

The alcohol dissolves lipids FOUND in the OUTER cell MEMBRANE of Gram-negative bacteria, allowing the crystal violet-iodine complex to leak out of the thinner peptidoglycan layer. The alcohol is added for 10 to 20 seconds; it is POURED on the slide until all the iodine is washed away and the run-off is COLORLESS



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