InterviewSolution
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InterviewSolution
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The most important feature in a plasmid to be used as a vector isA. origin of replication (ori)B. presence of a selectable markerC. presence of sites for restriction endonucleaseD. its size |
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Answer» Correct Answer - A::B::C::D Origin of replication (ori) a selectable marker, sites for restriction endonuclease and its size, all are important features required to facilitate cloning into a vector. A good DNA vector should be able to replicate autonomously in the host cell, for which it needs to have an origin of replication site (ori). This is important for the replication of inserted gene. A prokaryotic DNA has a single ori while eukaryotic DNA mat have more than one ori. Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of transformants. Cloning sites (recongnition sites) and the sites where the DNA is cut by a restriction endonuclease. A vector should be ideally lesss than 10kb in size because large. DNA molecules can break down during purification procedure. option (a) and (c) are absolutely necessary for it to act vector, while in absence of (b), the efficiency of overall procedure will decrease. |
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