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InterviewSolution
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The term "competent" refers toA. increasing the competition between cellsB. making cells impermeable for DNAC. increasing the efficiency with which DNA enters the bacterium through pores in its cell wallD. making cells permeable for divalent cations |
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Answer» Correct Answer - C Transformation is a process by which a cell takes up naked DNA fragment from the environment, incorporates it into its own chromosomal DNA and finally expresses tha trait controlled by the incoming DNA. Since DNA is a hydrophilic molecule, it can not pass through membranes, so the bacterial cells must be made competent to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium `(Ca^(2+))` which increases teh efficiency with which DNA enters the bacterium through pores in its cell wall. |
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