1.

What are sticky ends? State their significance in recombination DNA technology.

Answer»
  • Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. These overhanging stretches on each strand are called sticky ends.
  • They form hydrogen bonds with their complimentary counterparts and facilitate the action of DNA ligase enzyme.


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