1.

What are the Applications of PCR?

Answer»

Applications of PCR :

(1) Cloning a gene encoding a known protein 

(2) Amplifying 'old DNA' 

(3) Amplifying cloned DNA from vectors 

(4) Creating mutations in cloned genes 

(5) Rapid amplification of cDNA ends - RACE 

(6) Detecting bacterial or viral infection 

  • AIDs infection
  • Tuberculosis (Mycobacterium tuberculosis) 

(7) Cancer Detecting mutations that occur in cancer and monitoring cancer therapy. Determining if a patient is free of malignant cells 

(8) Genetic diagnosis 

a. Diagnosing inherited disorders 

b. Diagnosing cancer 

c. Blood group typing

d. Prenatal diagnosis



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