What are the different techniques and devices used in r-DNA technology

1.	What are the different techniques and devices used in r-DNA technology?
Answer» (1) Several techniques are used in r-DNA technology to isolate and characterize the macromolecules like DNA, RNA, proteins. (2) The techniques used on the basis of molecular weight are gel permeation, osmotic pressure, ion exchange chromatography, spectroscopy, mass spectrometry, electrophoresis, etc. (3) Electrophoresis: It is used for the separation of charged molecules like DNA, RNA and proteins, by application of an electric field. Different types of electrophoresis : Agarose gel electrophoresis, PAGE, SDA PAGE. (4) Polymerase chain reaction (PCR) : It is used for in vitro gene cloning or gene multiplication to produce a billion copies of the desired segment of DNA or RNA, with high accuracy and specificity, in few hours.

What are the different techniques and devices used in r-DNA technology?

Answer»

(1) Several techniques are used in r-DNA technology to isolate and characterize the macromolecules like DNA, RNA, proteins.

(2) The techniques used on the basis of molecular weight are gel permeation, osmotic pressure, ion exchange chromatography, spectroscopy, mass spectrometry, electrophoresis, etc.

(3) Electrophoresis:

It is used for the separation of charged molecules like DNA, RNA and proteins, by application of an electric field.
Different types of electrophoresis : Agarose gel electrophoresis, PAGE, SDA PAGE.

(4) Polymerase chain reaction (PCR) : It is used for in vitro gene cloning or gene multiplication to produce a billion copies of the desired segment of DNA or RNA, with high accuracy and specificity, in few hours.

Discussion

No Comment Found

Related InterviewSolutions

In rDNA technology the alein DNA and cloning vector are a cut by same Restriction endo nuclease enzyme. Give reason.
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is calledA. ElectrophoresisB. ResolutionC. ElutionD. Spooling
A typical agarose gel electrophoresis showing migration of undigested (lane 1) and digested set DNA fragments (lane 2 to 4) is show below. Identify A, B ,C and D by selecting the option : A. A-DNA bnads , B-Wells ,C-Largest , D-SmallestB. A-Wells , B-DNA bands , C-Smallest, D-LargestC. A-Wells , B-DNA bands , C-Largest, D-SmallestD. A-Smallest, B-Wells , C-DNA bands, D-Largest
Agarose extracted from sea weeds finds use inA. Gel electrophoresisB. SpectrophotometryC. Tissue cultureD. PCR
Agarose extracted from sea weeds finds use inA. PCRB. Gel electrophoresisC. SpectrophotometryD. Tissue culture
A mixture containing DNA fragments a, b, c and d with molecular weigths of `a+b=c,a gt b` and `d gt c`, was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anode sides of the gel would beA. B,A,C,DB. A,B,C,DC. C,B,A,DD. B,A,D,C
Which one is used as vector in genetic engineering ?A. PlasmidB. BacteriumC. CyanophageD. None of these
Agarose extracted from sea weeds finds use inA. Tissue cultureB. PCRC. Gel electrophoresisD. Spectrophotomery
Read the following four statements (A-D) A. The first transgenic buffalo, Roise produced milk which was human alpha-lactalbumin enriched B. Restriction enzymes are used in isolation of DNA from other macromolecules C. Downstream processing is one of the step of rDNA technology D. Disarmed pathogen vectors are also used in transfer of rDNA into the host which of the two statements have mistakes ?A. Statements (A) and (B)B. Statements(B) and (C)C. Statements (C) and (D)D. Statements (A) and (C)
Which one of the following techniques made it possible to gnetically engineer living organisms ?A. HybridizationB. Recombinant DNA techniquesC. X-ray diffractionD. Heavier isotope labelling