What are the features that a vector must possess to facilitate cloning

1.	What are the features that a vector must possess to facilitate cloning?
Answer» The following are the features that are required to facilitate cloning into a vector. 1. Origin of replication (ori): This is a sequence from where replication starts and piece of DNA when linked to this sequence can be made to replicate within the host cells. 2. Selectable marker: In addition to ori the vector requires a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants, 3. Cloning sites: In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes.

What are the features that a vector must possess to facilitate cloning?

Answer»

The following are the features that are required to facilitate cloning into a vector.

1. Origin of replication (ori): This is a sequence from where replication starts and piece of DNA when linked to this sequence can be made to replicate within the host cells.

2. Selectable marker: In addition to ori the vector requires a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants,

3. Cloning sites: In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes.

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