What are the principles behind Iso electric focusing and SDS PAGE tech

1.	What are the principles behind Iso electric focusing and SDS PAGE techniques? Why is 2D electrophoresis better than single dimension electrophoresis?
Answer» Principle of IEF: Separation of proteins on the basis of their different pI values Principle of SDS PAGE: Separation of proteins on the basis of their size 2D electrophoresis is better because proteins are separated into 2D patterns with high resolution on the basis of charge and size.

What are the principles behind Iso electric focusing and SDS PAGE techniques? Why is 2D electrophoresis better than single dimension electrophoresis?

Answer»

Principle of IEF:

Separation of proteins on the basis of their different pI values

Principle of SDS PAGE:

Separation of proteins on the basis of their size 2D electrophoresis is better because proteins are separated into 2D patterns with high resolution on the basis of charge and size.

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What are the principles behind Iso electric focusing and SDS PAGE techniques? Why is 2D electrophoresis better than single dimension electrophoresis?

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