What are the Quite straightforward to isolate DNA?

1.	What are the Quite straightforward to isolate DNA?
Answer» For instance, to isolate genomic DNA 1. Remove tissue from organism 2. Homogenise in lysis buffer containing guanidine thiocyanate (denatures proteins) 3. Mix with phenol/chloroform - removes proteins 4. Keep aqueous phase (contains DNA) 5. Add alcohol (ethanol or isopropanol) to precipitate DNA from solution 6. Collect DNA pellet by centrifugation 7. Dry DNA pellet and resuspend in buffer 100 8. Store at 4°C Each cell (with a few exceptions) carries a copy of the DNA sequences which make up the organism's genome.

Answer»

For instance, to isolate genomic DNA

1. Remove tissue from organism

2. Homogenise in lysis buffer containing guanidine thiocyanate (denatures proteins)

3. Mix with phenol/chloroform - removes proteins

4. Keep aqueous phase (contains DNA)

5. Add alcohol (ethanol or isopropanol) to precipitate DNA from solution

6. Collect DNA pellet by centrifugation

7. Dry DNA pellet and resuspend in buffer 100

8. Store at 4°C

Each cell (with a few exceptions) carries a copy of the DNA sequences which make up the organism's genome.

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