What do you mean by cytological fixatives and mention the properties o

1.	What do you mean by cytological fixatives and mention the properties of good cytological fixative.
Answer» It is critical to fix cytology specimens immediately after collection for proper preservation of the cellular components. It is important that no air-drying occurs prior to fixation. If a smear is already air-dried it should not be put in an alcohol fixative. Please note on the requisition if the slide (s) being submitted are fixed or air-dried. Properties of a good cytological fixative: • It should not excessively shrink or swell cells. • It should not distort or dissolve cellular components. • It should help preserve nuclear details. • It should improve optical differentiation and enhance staining properties of the tissues and cell components.

What do you mean by cytological fixatives and mention the properties of good cytological fixative.

Answer»

It is critical to fix cytology specimens immediately after collection for proper preservation of the cellular components. It is important that no air-drying occurs prior to fixation. If a smear is already air-dried it should not be put in an alcohol fixative. Please note on the requisition if the slide (s) being submitted are fixed or air-dried. Properties of a good cytological fixative:

• It should not excessively shrink or swell cells.

• It should not distort or dissolve cellular components.

• It should help preserve nuclear details.

• It should improve optical differentiation and enhance staining properties of the tissues and cell components.

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