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What Happens In The Flow Cytometer? |
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Answer» Cells go through CHAMBER and ideally PASS through 1–by–1. Laser light source shines on cells. If ABS (from reagent you added) are attached to cells they will give off their fluorochrome and you will SEE fluorescence. Detects forward scatter properties and SIDE scatter properties on different cells. Cells go through chamber and ideally pass through 1–by–1. Laser light source shines on cells. If Abs (from reagent you added) are attached to cells they will give off their fluorochrome and you will see fluorescence. Detects forward scatter properties and side scatter properties on different cells. |
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