What is DNA finger printing? Describe the various steps involved in th

1.	What is DNA finger printing? Describe the various steps involved in the technique of DNA fingerprinting.
Answer» DNA finger printing is a technique used for determining the nucleotide sequences of certain regions of DNA which are unique.to each individual and distinguishes him or her more exactly from others. Using DNA for distinguishing and identification of individuals is called DNA finger printing. It works on the principle of polymorphism in DNA sequences. Various steps involved the technique of DNA fingerprinting 1. The isolation of the desired DNA from small amounts of blood, semen or other cells of body. 2. The digestion of DNA by restriction endonuclease enzyme. 3. The blotting of separate DNA fragments to synthetic membrane e.g., nylon or nitrocellulose membranes. 4. The hybridization by use of labelled VNTR probe (mini satellite). 5. By autoradiography, the detection of hybridised DNA fragments is done.

What is DNA finger printing? Describe the various steps involved in the technique of DNA fingerprinting.

Answer»

DNA finger printing is a technique used for determining the nucleotide sequences of certain regions of DNA which are unique.to each individual and distinguishes him or her more exactly from others. Using DNA for distinguishing and identification of individuals is called DNA finger printing. It works on the principle of polymorphism in DNA sequences.

Various steps involved the technique of DNA fingerprinting

1. The isolation of the desired DNA from small amounts of blood, semen or other cells of body.

2. The digestion of DNA by restriction endonuclease enzyme.

3. The blotting of separate DNA fragments to synthetic membrane e.g., nylon or nitrocellulose membranes.

4. The hybridization by use of labelled VNTR probe (mini satellite).

5. By autoradiography, the detection of hybridised DNA fragments is done.

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