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What is the Hemoglobin and Heterogeneous ​

Answer»

Hemoglobin samples from NORMAL adult individuals, from newborn babies and from patients suffering from some hemoglobinopathies were studied by different analytical procedures. A close relation between the heterogeneities found by starch block ELECTROPHORESIS and by chromatography on CM-cellulose as well as on Amberlite IRC-50 was demonstrated.

The hemoglobin of a normal adult individual was found to consist of six Hb-fractions named Hb-A1A, Hb-A1B, Hb-A1C Hb-A0 Hb-F and Hb-A2, while next to these fractions a slightly yellow fraction was found consisting of the methemoglobin reductase (about 10 %) and other unknown PROTEINS. Some data on the Chromatographic behaviour of the recently discovered abnormal Hb-A2 fraction (named Hb-A2') are also presented.

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