What is the meaning of sterilisation? Why is it essential in tissue cu – InterviewSolution

1.	What is the meaning of sterilisation? Why is it essential in tissue culture?
Answer» Sterilisation refers to the complete killing of microorganisms so that a normal, pathogen free condition is created. Sterilisation is essential in tissue culture because it requires regeneration of plant from explants, in a test tube, under sterile conditions. So, sterilisation is prerequisite as growth of unwanted microorganisms would contaminate the nutrient media and further procedure would be difficult to carry out.

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