1.

What is tissue culture? What are the different types of tissue culture? Describe briefly.

Answer»

The production of new plants from a small piece of plant tissue (or cells) removed from the  growing tips of a plant in a suitable growth medium is called tissue culture.

The different types of tissue culture is as follows:

1. Seed Culture: Seeds may be cultured in vitro to generate seedlings or plants. It is the best method for raising the sterile seedling. The seed culture is done to get the different kinds of explants from aseptically grown plants which help in better maintenance of aseptic tissue.

2. Embryo Culture: Embryo culture is the sterile isolation and growth of an immature or mature embryo in vitro with the goal of obtaining a viable plant. In some plants seed dormancy may be due to chemical inhibitors or mechanical resistance, structures covering the embryo. Excision of embryos and culturing them in nutrient media help in developing viable seedlings.

3. Bud Culture: Buds contain quiescent or active meristems in the leaf axils, which are capable of growing into a shoot. Single node culture, where each node of the stem is cut and allowed to grow on a nutrient media to develop the shoot tip from the axil which ultimately develops into new plantlet. In axillary bud method, where the axillary buds are isolated from the leaf axils and develop into shoot tip under little high cytokinin concentration.

4. Callus Culture: Callus is basically more or less un-organised dedifferentiated mass of cells arising from any kind of explant under in vitro cultural conditions. The cells in callus are parenchymatous in nature, but may or may not be homogenous mass of cells. They are meristematic tissue, under special circumstances they may be again organised into shoot primordia or may develop into somatic embryos.

The callus tissue from different plant species may be different in structure and growth habit. The callus growth is also depen­dent on factors like the type of explant and the growth conditions. After callus induction it can be sub-cultured regularly with appropriate new medium for growth and maintenance.



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