Which methodology is used while sequencing the total DNA from a cell?

1.	Which methodology is used while sequencing the total DNA from a cell? Explain it in detail.
Answer» Methodology used: • Sequence Annotation – total DNA from a cell is isolated, converted into random fragments of relatively smaller sizes, and cloned in suitable host using specialized vectors. • The cloning resulted into amplification of each piece of DNA fragment. • The fragments were sequenced using automated DNA sequencers, these sequences are then arranged based on some overlapping regions (present in them). • This requires generation of overlapping fragments (for sequencing). • Specialised computer based programmes were developed, and these sequences were subsequently annotated and assigned to each chromosome.

Which methodology is used while sequencing the total DNA from a cell? Explain it in detail.

Answer»

Methodology used:

• Sequence Annotation – total DNA from a cell is isolated, converted into random fragments of relatively smaller sizes, and cloned in suitable host using specialized vectors.

• The cloning resulted into amplification of each piece of DNA fragment.

• The fragments were sequenced using automated DNA sequencers, these sequences are then arranged based on some overlapping regions (present in them).

• This requires generation of overlapping fragments (for sequencing).

• Specialised computer based programmes were developed, and these sequences were subsequently annotated and assigned to each chromosome.

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