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Which of the following centrifugation is used to separate certain organelles from whole cell?(a) Rate-zonal centrifugation(b) Normal centrifugation(c) Differential centrifugation(d) Isopycnic centrifugation |
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Answer» Right option is (c) Differential centrifugation Easy explanation: The simplest form of separation by centrifugation is differential centrifugation, sometimes called differential pelleting. Particles of different densities or sizes in a suspension will sediment at different rates, with the larger and denser particles sedimenting faster. These sedimentation rates can be increased by using centrifugal force. A suspension of cells subjected to a series of increasing centrifugal force cycles will yield a series of pellets containing cells of decreasing sedimentation rate. Differential centrifugation is a common procedure in microbiology and cytology used to separate certain organelles from whole cells for further analysis of specific parts of cells. |
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