Which of the following steps are catalysed by Taq polymerase in a PCR reaction ?A. Denaturation of template DNAB. Annealing of primers to template DNAC. Extension of primer and on the template DNAD. All of the above

Which of the following steps are catalysed by Taq polymerase in a PCR

1.	Which of the following steps are catalysed by Taq polymerase in a PCR reaction ?A. Denaturation of template DNAB. Annealing of primers to template DNAC. Extension of primer and on the template DNAD. All of the above
Answer» Correct Answer - C The final step of PCR is extension, wherein Taq DNA polymerase (isolated from a thermophilic bacterium Thermus aquaticus) synthesises the DNA region between the primers, using dNTPs (denoxynucleoside triphosphates) and `Mg^(2+)`. The primers are extended towards each other so that the optimum temperature for this polymerisation step is `72^(@)C`. Taq polymerase remains active during high temperature induced denaturation of double stranded DNA.

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Which of the following steps are catalysed by Taq polymerase in a PCR reaction ?A. Denaturation of template DNAB. Annealing of primers to template DNAC. Extension of primer and on the template DNAD. All of the above

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