1.

Which of the following steps during glycolysis is associated with utilisation of ATP?A. Glucose `rarr` Glucose -6-phosphateB. Fructose-6-phosphate `rarr` Fructose-1,6-biphosphateC. PEP `rarr` Pyruvic acidD. Both (a) and (b)

Answer» Correct Answer - D
In the initial phase (energy spendling phase) of glycolysis, the hexoses (i.e., glucose and fructose) ar phosphorylated in a reaction that uses ATP at two steps : First in the conversion of glucose into glucose-6-phosphate and second in the conversion of fructose-6-phophaste to fructose-1,6-biphosphate.
These reactions are catalysed by the hexokinase and phospho-fructokinase respectively
`"Glucose + ATP" underset(Mg^(2+))overset("hexokinase")(rarr) "Glucose-6-phosphate + ADP"`
`"Fructose-6-phosphate + ATP" underset(Mg^(2+))overset("Phosphofructokinase")(rarr) "Fructose-1,6-biphosphate + ADP"`


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